Estimation of Nifedipine in Human Plasma by Lcms/ms
نویسندگان
چکیده
Nifedipine and NifedipineD6, (IS) were extracted from the plasma by precipitation method and then separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min. The analytes were detected in API 4000 Mass spectrometer in the positive atmospheric pressure chemical Ionization (APCI) mode with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 347.2& daughter ion 315.1(Nifedipine), and m/z 353.2& daughter318.2 (Nifedipine D6, IS). A linear calibration plot of Nifedipine was achieved in the concentration ranges of 1.558 ng/mL to 360.561ng/mL. Mean recovery was93.2%. The assay was specific, precise, accurate and reproducible.
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